Scientific physical exercise in maintaining health / Exercício físico científico na manutenção da saúde / Ejercicio físico científico para mantener la salud

ABSTRACT Introduction According to the 2015 National Physical Health Monitoring Report, most of the national physical health indicators have begun to rebound, but some people's physical health is still declining. Object The thesis studies the problems existing in people's physical exercise and guides the development of these people's habits. Methods Our mathematical statistics and other research methods investigate the current situation of people's physical exercise habits, and explore the factors that restrict habits from the factors that affect the formation of sports and fitness concepts. Result The proportion of people developing physical exercise habits is low. People invest less time and energy in physical exercise. Conclusion The less time and energy that people invest in physical exercise is the main reason that affects their belief in exercise and fitness and physical exercise habits. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMO Introdução De acordo com o Relatório Nacional de Monitoramento de Saúde Física de 2015, a maioria dos indicadores nacionais de saúde física começou a se recuperar, mas a saúde física de algumas pessoas ainda está em declínio. Objetivo a tese estuda os problemas existentes no exercício físico das pessoas e orienta o desenvolvimento dos hábitos dessas pessoas. Métodos Nossas estatísticas matemáticas e outros métodos de pesquisa investigam a situação atual dos hábitos de exercício físico das pessoas e exploram os fatores que restringem os hábitos e os fatores que afetam a formação de conceitos de esportes e preparação física. Resultado a proporção de pessoas que desenvolvem hábitos de exercícios físicos é baixa. As pessoas investem menos tempo e energia em exercícios físicos. Conclusão O pouco tempo e energia que as pessoas investem na prática de exercícios físicos é o principal motivo que afeta sua crença na prática de exercícios e hábitos de exercício físico. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción De acuerdo con el Informe Nacional de Monitoreo de la Salud Física de 2015, la mayoría de los indicadores nacionales de salud física han comenzado a recuperarse, pero la salud física de algunas personas todavía está en declive. Objetivo la tesis estudia los problemas existentes en el ejercicio físico de las personas y orienta el desarrollo de los hábitos de estas personas. Métodos Nuestras estadísticas matemáticas y otros métodos de investigación investigan la situación actual de los hábitos de ejercicio físico de las personas y exploran los factores que restringen los hábitos y los factores que afectan la formación de conceptos deportivos y la preparación física. Resultado la proporción de personas que desarrollan hábitos de ejercicio físico es baja. La gente invierte menos tiempo y energía en el ejercicio físico. Conclusión El poco tiempo y energía que las personas invierten en la práctica de ejercicio físico es el principal motivo que incide en su creencia en la práctica de ejercicio y hábitos de ejercicio físico. Nivel de evidencia II; Estudios terapéuticos: investigación de los resultados del tratamiento.

Effect of silencing FBXO2 expression on proliferation, invasion, migration, and EMT of gastric cancer cells / 中国肿瘤生物治疗杂志

@#［摘要］ 目的：探讨F 框蛋白2（F-box only protein 2，FBXO2）基因在人胃癌细胞系中表达及其对胃癌细胞增殖、迁移、侵袭和 EMT 的影响。方法：选择胃癌细胞系MGC-803、AGS、SGC-7901、MKN-28 以及正常胃黏膜上皮细胞株GES-1，qPCR 法检测细 胞中FBXO2 mRNA表达水平。设计靶向抑制FBXO2 表达的特异siRNA，并瞬时转染MGC-803 细胞，转染siRNA无义序列的为 阴性对照。qPCR法检测转染48 h 后MGC-803 细胞中FBXO2 mRNA表达水平；用MTT法、细胞划痕愈合法、Transwell 小室法检 测降低FBXO2 表达对细胞增殖、迁移和侵袭的影响，WB 法检测细胞中EMT 相关蛋白E-cadherin、N-cadherin、vimentin 的表达。 结果：4 种胃癌细胞中FBXO2 mRNA表达水平显著高于胃黏膜上皮细胞GES-1（P<0.05 或P<0.01）。与阴性对照组相比，siRNAFBXO2 组MGC-803 细胞中FBXO2 mRNA表达下调（P<0.01），该细胞的增殖、迁移和侵袭能力受到显著抑制（P<0.05 或P<0.01）， E-cadherin 蛋白表达明显升高（P<0.01），N-cadherin、vimentin 蛋白表达显著降低（均P<0.01）。结论：低表达的FBXO2 可抑制胃 癌细胞的增殖、迁移和侵袭能力，该抑制作用可能与EMT过程有关。

Significance of Mycobacterium tuberculosis antigen expression in cerebrospinal fluid monocytes in diagnosing tuberculous meningitis.

Background: The aim of this study was to investigate the signifi cance of positive expression of Mycobacterium tuberculosis, (MTB) antigen in the cerebrospinal fl uid (CSF) monocytes in diagnosing tuberculous meningitis (TBM). Materials and Methods: A total of 50 inpatients of TBM, 30 viral meningitis and 20 healthy controls were studied at the 1st, 2nd, and 4th week during their treatment course. Immunohistochemical assay were used to detect early secreted antigenic target 6 (ESAT-6) positive cells, and positive cases were also observed. Results: The percentage of positive cases and positive cells of ESAT-6 in CSF monocytes were all higher in the 1st and 2nd week than in the 4th week in TBM patients (P < 0.01); and percentage of positive cases and positive cells of MTB antigen in CSF monocytes were higher in TBM patients than in viral meningitis and health control in the 1st and 2nd week (P < 0.01). The sensitivity was 90% and the specifi city was 92% in the early stage (within 2 weeks) of TBM. Conclusion: The positive expression of ESAT-6 in CSF monocytes is helpful for the early diagnosis of TBM.

Inhibitory effect of α-tocopherol on methylmercury-induced oxidative steress

<p><b>OBJECTIVES</b>The present study investigated the involvement of oxidative stress in the degeneration of the cerebellum during methylmercury (MeHg) intoxication and the protective effect of α-tocopherol (Vit E) against MeHg toxicity.</p><p><b>METHODS</b>After 5 mg/kg of MeHg was administered to Wistar rats for 12 consecutive days, the cerebellum were examined histopathologically. In addition, the same amount of MeHg was administered to 3 different groups of Wistar rats: rats with a Vit E-deficient diet, rats fed 150 mg/kg of Vit E for 20 consecutive days after initial MeHg administration, and rats with an ordinary diet.</p><p><b>RESULTS</b>Positive immunoreactivity against anti-hydroxynonenal (HNE), a marker of lipid peroxidation, was observed in the cerebellum after MeHg administration. Levels of thiobarbituric acid reactive substance (TBARS), another marker of lipid peroxidation, and those of protein carbonyl, a biomarker for protein oxidation, increased after MeHg administration. In the rats with MeHg and a Vit E-deficient diet, mortality and prevalence of piloerection significantly increased, and in the rats with MeHg and Vit E, mortality, piloerection, retracted and crossed hind leg, and ataxic gait significantly decreased, compared with the rats with MeHg alone. The levels of NO(2) (-) and NO(3) (-) in the serum significantly increased in the rats with MeHg alone 14 days after the initial MeHg administration, but were significantly suppressed by Vit E administration.</p><p><b>CONCLUSIONS</b>Oxidative stress, especially lipid peroxidation, may play an important role in the cerebellar degeneration process during MeHg intoxication and Vit E may play a protective role against MeHg toxicity as an effective antioxidant.</p>

Inhibitory Effect of $\alpha$-Tocopherol on Methylmercury-Induced Oxidative Steress

Objectives: The present study investigated the involvement of oxidative stress in the degeneration of the cerebellum during methylmercury (MeHg) intoxication and the protective effect of α-tocopherol (Vit E) against MeHg toxicity. Methods: After 5 mg/kg of MeHg was administered to Wistar rats for 12 consecutive days, the cerebellum were examined histopathologically. In addition, the same amount of MeHg was administered to 3 different groups of Wistar rats: rats with a Vit E-deficient diet, rats fed 150 mg/kg of Vit E for 20 consecutive days after initial MeHg administration, and rats with an ordinary diet. Results: Positive immunoreactivity against anti-hydroxynonenal (HNE), a marker of lipid peroxidation, was observed in the cerebellum after MeHg administration. Levels of thiobarbituric acid reactive substance (TBARS), another marker of lipid peroxidation, and those of protein carbonyl, a biomarker for protein oxidation, increased after MeHg administration. In the rats with MeHg and a Vit E-deficient diet, mortality and prevalence of piloerection significantly increased, and in the rats with MeHg and Vit E, mortality, piloerection, retracted and crossed hind leg, and ataxic gait significantly decreased, compared with the rats with MeHg alone. The levels of NO2− and NO3− in the serum significantly increased in the rats with MeHg alone 14 days after the initial MeHg administration, but were significantly suppressed by Vit E administration. Conclusions: Oxidative stress, especially lipid peroxidation, may play an important role in the cerebellar degeneration process during MeHg intoxication and Vit E may play a protective role against MeHg toxicity as an effective antioxidant.